DIFFERENTIAL INHIBITION OF ESTERASE ISOZYMES BY ORGANOPHOSPHORUS COMPOUNDS
نویسندگان
چکیده
منابع مشابه
The inhibition of hydrolytic enzymes by organophosphorus compounds.
It has been shown that several hydrolytic enzymes with different specificities undergo similar reactions with diisopropyl phosphorofluoridate (1) and other related toxic organophosphorus compounds (2). These conclusions were based on kinetic studies, particularly the influence of pH on the rates of reaction, and on inhibition characteristics. Reactions of this type have provided a unique method...
متن کاملThe mechanism of inhibition of cholinesterases by organophosphorus compounds.
1. The soluble and insoluble fractions of eight species of bacteria were separated by centrifuging the material obtained after disintegrating the cells by shaking with glass beads. 2. The insoluble material, believed to be at least mainly derived from the cell wall, accounted in most cases for about half of the dry weight of the cell. 3. Estimations were performed on both fractions for ash, nit...
متن کاملHydrolysis of organophosphorus compounds by an esterase isozyme from insecticide resistant pest Helicoverpa armigera.
Esterase activity of resistant and susceptible H. armigera were compared in gels with different substrate such as naphthyl acetate, naphthyl phosphate, paraoxon and monocrotophos. Whole body extract of resistant H. armigera hydrolyzed paraoxon, monocrotophos and naphthyl phosphate in gels. Resistant H. armigera showed high esterase, phosphatase and paraoxon hydrolase activity compared to suscep...
متن کاملDetection of organophosphorus compounds by covalently immobilized organophosphorus hydrolase.
As a consequence of organophosphorus (OP) toxins posing a threat to human life globally, organophosphorus hydrolase (OPH) has become the enzyme of choice to detoxify such compounds. Organophosphorus hydrolase was covalently immobilized onto a quartz substrate for utilization in paraoxon detection. The substrate was cleaned and modified prior to chemical attachment. Each modification step was mo...
متن کاملThe delayed neurotoxic effect of some organophosphorus compounds. Identification of the phosphorylation site as an esterase.
1. Organophosphorus compounds that produce a delayed neurotoxic effect in hens phosphorylate a specific site in the brain soon after administration. 2. Phosphorylation of the specific site by di-isopropyl [(32)P]phosphorofluoridate in vitro is blocked by the prior addition of phenyl phenylacetate. 3. A small proportion of the total activity of hen brain hydrolysing phenyl phenylacetate in vitro...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1971
ISSN: 0022-1554,1551-5044
DOI: 10.1177/19.6.390